During evolutionary process, plants adapted to gradual climatic changes and they acquired potential defense mechanisms, including low molecular weight compounds, proteins, and peptides exhibiting antimicrobial activities. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.Īccording to Selitrennikoff, in every ecological system about 250,000 fungi are generally distributed and certain microorganisms are able to produce severe damaging effect on quality as well as production of important crop plants. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. Ice crystallization primarily damages frozen food items however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus however combined effect of both agents maximized activity against the pathogen. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. Basrai antifungal TLP was tried as pharmacological agent against fungal disease.
Western blot analysis showed the presence of approximately 20 kDa protein in induced cells.
Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37☌. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli.
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